Spectrophotometer and Microplate Reader

A lot of people would like to know the difference of the Microplate reader and the Spectrophotometer when they request the Spectrophotometer.But after knowing eliasa, Most of them will choose to buy our Microplate reader.Now I want to introduce the similarities and difference between them.Hope it can help you how to choose.
According to the function different division,Eliasa can be divided into (1) light absorption microplate reader (visible microplate reader, ultraviolet/visible microplate reader) (2) fluorescence microplate reader (3) chemiluminescence microplate reader.
In accordance with the wavelength and application fields,Spectrophotometer can be divided into: (1) visible spectrophotometer (2) ultraviolet spectrophotometer (3) infrared spectrophotometer (4) fluorescence spectrophotometer (5) atomic absorption spectrophotometer.
Spectrophotometer and eliasa are laboratory commonly used two kinds of instruments, their measurement principle is the same.All use Lambert-Beer law.All measure the sample's absorbance.
There are some differences between Spectrophotometer and microplate reader. Specific differences reflected in the following three aspects:
(1) measuring solution containers:
Spectrophotometer uses cuvettes, microplate reader is using plastic micropore board (enzyme label plate). Cuvettes can only play the role to contain solution, every cuvettes can only contain a kind of solution. Enzyme label plate commonly used transparent polyethylene materials,It has strong adsorption to antigen antibody.So it used as a solid carrier. Enzyme label plate is usually 48 holes or 96 holes, every micro hole can contain different solution.
(2) the light path direction:
Spectrophotometer is level light path, and eliasa is vertical light path. Due to the enzyme label plate holes is muilti rows of porous, light can only vertical go through. So eliasa beam vertical go through the test to solution and micro hole plate.The beam is not only from top to bottom, but also can be from the bottom up through the color solution. Vertical light's characteristic is that the specimen absorbance by liquid enrichment or diluting effect is small.The deficiency is that it will be affected a lot by measured sample liquid level whether level, enzyme label plate pervious to light quality, the bottom of the hole whether level and so on.
(3) optical path length:
Since light density (OD value) and absorption coefficient, to test concentration of components and optical path length is proportional to the relationship.
The width of color cup used by Spectrophotometeris usually 1 cm, so light path length fixed to 1 cm. Therefore different instruments, different batch measurement data has the same comparability.
And eliasa is the vertical light path, so light path length should be liquid levels. So the value of measured influnced by sample volume.
Anyway,Our mircroplate reader DNM series and Microplate washer DNX series with 48 wells micro plates and 96 wells micro plates will supply you better test experience and they cost effective.Pls feel free to contact us if you want to know more details of our microplate readers.We will do our best to support you and bring you a happy purchasing and using experience.We are awaiting to hearing from you and give you better service.