Eliasa test method (ELISA) what is the principle?

We go to hospital for a scan in the used equipment has its principle of work place, if working staff to understand the medical equipment work principle can better use instrument. Also can help workers better finish the work. Microplate reader is our life is very wide application of medical equipment, the eliasa immune adsorption test explain the basic principle of:

(a) to excess specific antibody or antigen) envelope in carrier (enzyme label plate), through the antigen antibody response to side substances (antigen or antibody) and enzyme marker (with HRP - horseradish peroxidase mark antibody or antigen) also combined with the carrier (solid phase separation), the washing to remove free enzyme marker, then add the substrate, has combined with the carrier to mark enzyme substrate color. Finally using photoelectric colorimetry, treat measuring content qualitative judgment or quantitative determination.
The above methods inspection sensitivity can achieve ng/ml level.

(2) envelope enzyme label plate: specific antigen solution to join enzyme label plate hole, 4 ℃ for the night. Then use liquid detergent washing 3 to 5 times (washing machine wash plate), this solution of specific antigen was strong adsorption in enzyme label plate micro hole surface, form solid phase antigen, residual liquid and impurity were washed away.

(3) to join measured sample liquid (patient serum) : sample in liquid if contains the remains to be measuring antibody, after incubate reaction, criterion and already envelope of antigen produce specific combination, generating coating antigen and antibody test to the complex, adsorbing in elisa reader analyzer  micro hole surface. Then again for washing, remove residual liquid and interfering substance.

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High Frequency Mobile C-arm System hot sale

This is high frequency mobile C-arm system, It is widely used in orthopedics surgery, diaplasis, cardiac catheter, implanting pack maker, interventional therapy, partial radiography, local photography, and other work.
The power output is 5kw, main inverter frequency is 40kHz, tube voltage is 40~120kv, for film the tube current is 20-100mA, For fluoroscopy we have three pattems: automatic fluoroscopy, manual fluoroscopy and full pulse fluoroscopy. The automatic fluoroscopy the tube voltage is 40~120kv, the tube current is 0.3-40mA. The manual fluoroscopy, the tube voltage is 40~120kv, the tube current is 0.3-4mA. As for full pulse fluoroscopy, the tube voltage is 40~120kv, the tube current is 4.1-30mA. Especially for pulse fluoroscopy, the skin dose is lower but the image is much clearer. The tube type is fixed anode with dual-focus, small one is 0.3 and large one is 1.5.
This is leading domestic level mobile surgical X-ray machine. Convenient connection with the digital subtraction system. World-class image processing technology software makes the image more distinct to make it more convenient for the doctors in the operation and diagnostic process. Standard Dicom interface makes it convenient to connect with the hospital information system. The pulse fluoroscopy can obtain high-definition image from low close, low radiation close used to protect the doctor and patient.
Then let’s look at the control part.unique double foot brake contrlloer design makes it convenient to control the instryment inside and outside the operation room. This design can also protect the doctor health. Exclusive inside and outside monitor design makes it more convenient to observe the operation process either inside or outside the operation room. At the same time, this design can facilitate the outdoor teaching and the real-time observation of the surgical procedures.
Exclusive inside and outside monitor design makes it more convenient to observe the operation process either inside or outside the operation room. At the same time, this design can facilitate the outdoor teaching and the real-time observation of the surgical procedures.

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Five points of clinical biochemical analyzer reagents

Clinical biochemistry analyzer reagent such medical equipment  is the same as unusual consists of reagent storage and dispatch dosing device. About five clinical biochemical analyzer, as follows:
The reagent positions often and reagents dial from the combines.
Most of the clinical biochemistry analyzer reagent position is set to the fresh food compartment, in order to progress in line solid phase reagents.
Distribution of dosing device.
System identical with the sample, reagent probe is usually on the reagent pre-warmed probe the starting amount of the two-reagent system reagent 2 (R) should be lower, in order to jointly different R1 / R two ratio of the reagents.
Reagent bottle.
There are different shapes and not so big specifications. Should be based on something variable and reagents specifications. Ponder the residual reagent bottle died volume and exchange frequency, fair selection. Unique program cassette kit, small size, anti-evaporation, and convenience store.
Ancillary reagents often pen barcode.
Clinical biochemical analyzer features a pen barcode checking system, types of reagents, batch number, stock, validity and calibration curve cargo assassination, held in check verification, such as BeckmanCX seven.
Fifth, the reagent bottle cap open active dry system more conducive reagents save. Someclinical biochemical analyzer can be added in the run, exchange reagents, some needs to be held in the stopped state.

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What are the advantages of the dual channel hematology analyzer?

Hematology analyzer detection channel is divided into: single-channel hematology analyzer and a dual-channel blood cell analyzer. Two different channel hematology analyzer medical devices What difference does it make? Specific contents are as follows:
"Dual channel hematology analyzer first detection speed faster than single channel to more than double, which is a big advantage of the dual channel;
Another is to avoid plugging holes, blood leukocytes, erythrocytes, hemoglobin, platelet size selection according to the head of the channel by the popular parlance is "large cells can be passed from one channel, small cell from another channel through avoid a channel after blocking the passages of the large cell-small cell will not pass, causing blockage detection process fails.
Dual channel hematology analyzer another advantage is that you can selectively detect the leukocyte number or the number of red blood cells, reducing the detection time and cost needed to detect some of the items.
Dual channel hematology analyzer medical career development of emerging product, it generates is inevitable, and the emergence of dual channel hematology analyzer instead of the single-channel era.
The new generation of blood cell analyzer with dual-channel detection speed to avoid plugging holes. Has been widely applied to hospitals and health centers, community service centers, clinics and other medical institutions. All doctors have been known as "the doctor must have" instrument.

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What is Electrolyte analyzer common fault processing method?

Electrolyte analyzer is the hospital clinical examination the indispensable important medical devices, electrolyte analyzer in the clinical is mainly used to test maintain human blood. The blood osmotic pressure balance, in the surgery, burns, diarrhea, acute myocardial infarction and so on need a large number of equilibrium fluid infusion of the patients, the use of electrolyte analyzer to ion of testing and detection is very important. The precision and accuracy of the instrument is very high, for any sample measured results extremely accurate, reliable, fast speed, the operation is very simple. Sample can be whole blood, serum and plasma, urine, dialysis fluid, and hydration liquid.
Electrolyte analyzer to hospital so important, that if in use process appeared fault? For some common fault to how to deal with? The medical devices perlong small knitting to be a solution.
Electrolyte analyzer common fault processing methods:
A dull, suction sample, of the causes and treatment methods:
Suction sample impeded the major cause of basically has the following four, from the simple to the complex of thought to check;
1, check the line of each interface (including electrode between the electrode and even pipe, valve, between electrode and between handle) even tube if there is a gas leak, this phenomenon were not suction sample;
2, check the handle adhesion or too fatigue, if have, should be replaced new pump pipe. Phenomenon for the performance of the pump pipe issued abnormal sound;
3, each in the piping the layup if there is a protein precipitation, this kind of phenomenon for the performance of the fluid flow velocity process positioning instability, even in the new pump line also is same, solution to take down the joint water clean.
4, should also pay attention to is valve, carefully check.
Second, when there is detector failure is how to solve the:
The cause of the failure detector has the following kinds:
1, detector of plug and main board seat loose;
2, detector is broken;
3, valve core fixed screw and motor axis of rotation enough fastening;
4, valve core itself cannot turn too tight. Check the order of sequence for (3) - (1) - (4) - (2).
Three, electrode drift and the out of control causes and treatment methods:
1, electrode drift of the most common reason is ground missed, so should check ground; Check the drift of electrode silver bar is not insert signal socket or bad contact;
2, if electrode all drift, it shall check the reference electrode date is due;
3, positioning is bad, cause solution not all immersion electrode, should be reappraised positioning operation;
4, reference electrode above have bubbles, should pat flow cell, will bubble moved to Na electrode above;
5, voltage instability, had better pick UPS uninterruptible power supply or quality better regulated power supply;
6, avoid electromagnetic interference, power larger equipment should be as far as possible from the instrument, independent set up power;
7, inspection standard liquid and cleaning is finished;
8, check circulation pool reference filled with liquid is too little, and timely fill,
9, Na, pH electrode drift application in glass electrode cleaning fluid cleaning. Garnish with distilled water is rinsed repeatedly can;
10, reagent expired or polluted, check A and B standard liquid and cleaning fluid bottle, whether to have flocks.
Four, test blood sample abnormal value reason and solution:
When the test of blood samples, if abnormal value, according to the following steps to check:
1, whether there is a high power electric start near or leakage (such as: refrigerators), cause a voltage fluctuation;
2 at the time of the tests, suction clotting;
3, solution is not in place, can check whether the position is good, if the solution in place is not good, usable service program repositioning program for repositioning;
4, check put blood vessel is pollution, whether residual the disinfection of substance;
5, check correction factor is correct, whether to have abnormal phenomena, such as a anomaly, the correction factor cleared;
6, whether long time not calibration, the calibration can be measured again after again.
Five, the electrode slope reduce, processing method is as follows:
Electrode slope low, can cause test linear bad, sometimes will affect electrode repeatability, the main reasons are as follows:
1, temperature is too low,
2, life maturity;
4, air humidity;
3, electrode film board adsorption protein too much.
The contents of the article by perlong medical instrument company sort published, reprint please indicate the source!

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Full wavelength microplate reader offer price | full wavelength microplate reader manufacturers

Full wavelength elisa reader is extremely used throughout the country, because of the instrument performance, stability, and accuracy of data results, more importantly. The instrument has a robust detection function. Its range of applications across a variety of industries. The most commonly used in the food industry and the medical industry.
     Full wavelength microplate reader offer price how much?
     The Perlong Medical is a professional production of full wavelength microplate reader manufacturers and manufacturers. The Prang medical production of full wavelength microplate reader instrument measurement accuracy, good repeatability, quality control function, no external computer can automatically provide 31 days QC data and X, SD, CV values; the instrument preset operating procedures, test results can be stored; boot after the self-test function, to ensure the instrument each time work in normal working condition; and a wide range of instruments to measure the performance indicators have reached the international advanced level. Color LCD with touch-screen operation, can do genetic testing, display calibration curve advantages.
The following brief full wavelength microplate reader often malfunction and processing methods.
Phenomenon of a fault
The washer head clogging:
Failure Analysis and Maintenance: sample fibrin. Instrument self-test or washer process. Instrument self-test, can shut down the washer head treatment, re-POST: If the fault is in the washer process, you need to continue to press the "pause" key of the instrument, processing washer head wash board.
Processing method: Remove the washer head. Careful observation of each of the liquid injection port, and a liquid suction port. If you found a fibrin clot. Picked to go with a disposable syringe needle fibrin clot, and then clean the washer head; found no fibrin clot. Can be directly on the the Microplate head cleaning. When cleaning the washer head 5ml disposable syringe without needle, draw the the total liquid suction port of the distilled water alignment Xiban head or total fluid intake. Blocked another exit, the liquid inside the disposable syringes forcibly discharged. Total fluid outlet or the total fluid intake alternately repeated washing, to complete the cleaning of the Microplate head clogging. When the temperature is low. 5O ℃ 6o ℃ distilled water heated to rinse the effect is more obvious.
Symptom 2
Sample syringe and silicone tube junction leakage or off:
Failure Analysis and Maintenance: Because the sample needle clogging or liquid waste pipe is blocked, resulting in increased pressure of the pipeline, the silicone tube to break or fall off. Instrument self-test or the test process.
Processing methods: to stop instrument self-test or test, remove needle, very fine copper wires inside Tonga syringe. Check the silicone tube if there is rupture phenomenon, replace broken, connection. After the passage of the power-on self-test instrument pipeline flush. Troubleshooting, start the test, twice a week to remove the sample needle soaked in 5% ~ 1O% sodium hypochlorite solution for 2h and pipe with a 5% sodium hypochlorite solution rinse instruments. You can reduce the occurrence of this failure.
The failure phenomenon POST Times reagent disk error. The reagent disc kept turning, unable to stop.
Fault Analysis and Maintenance: This is caused due to the reagent disc underside of a small magnet off. The analyzer reagent disk using magnetic sensors to locate, at the bottom surface of the reagent disk set with the L small pieces magnet. Sensor fitted in the instrument underlying the above position reagent tray lined with small magnets to the above the sensor, the sensor will send a signal to locate the reagent disc, so the instrument self-test will pass.
Processing methods: shutdown. Picked reagent disk, reinstall the small magnet back, clean the sensor. The power-on self-test, the machine is operating normally.
Through the introduction of the above content, I believe you have some understanding of the full wavelength elisa reader, especially the instrument failed after processing. The Pulang medical production of medical device products, superior quality. Including: wavelength microplate reader, hematology analyzer, urine analyzer, biochemistry analyzer, coagulation analyzer, washer, hemoglobin analyzer and other equipment.
If you have any questions or you want to consult the full wavelength microplate reader offer price, you can call the the the Prang medical free hotline :400 -6656-888 contact our customer service staff. We are happy to serve you.

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Microplate reader so important to our life

Food safety is really a is not only to fill with righteous indignation, and tired of aversive topic, the but again absolutely can't cover your nose round. After all, it is each person's health life and death, even for the safety of the state and society.

In this important problem, experts special research and produce a kind of magical medical equipment, this kind of equipment application and extensive, and the test result is very precise, is about food safety related departments preferred medical devices, this kind of magical instrument equipment exactly is what? It is microplate reader. Believe that a lot of the industry insiders have heard this kind of instrument and equipment, but never really see it the magic of a place, have the opportunity to everybody should be a practical operation.

Perlong medical company is located in jiangsu province, nanjing, jiangning district, the company produces the microplate reader can be used in all parts of the country hospitals and relevant departments enzyme linked immunology testing work, the enzyme linked immunosorbent experimental hematology, immunology, tumor immunology, infectious disease immunology, eugenic and superior nurture of serology and genetic experiments, animal residual, residual pesticide residue, medicine, food, antibiotics, allergens, melamine and so on the many kinds of project detection.
The melamine is particularly important, "melamine" is a kind of triazine class nitrogen heterocyclic organic compound, used for chemical raw materials. White crystal, tasteless, slightly soluble in water, harmful to human body, do not used for food processing or food additives. But melamine diagnosis device can be in the scope of the provisions of the state class to add in milk powder and milk and other dairy products. If the adding amount of human body after too much smoking can lead to kidney stones and kidney failure cause health concerns. For example, once happened mengniu milk powder incident, fujian produce long rich pure milk events, and SanLu powdered milk melamine event and so on. Deeply hurt people's heart, making the image and status of the domestic milk powder suffer a disastrous decline. Thus foreign milk powder a lot of occupation of the Chinese market.

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