the study of the method of enzyme activity : enzyme from the inspection workstation biochemical analyzer determine

Perlong medical report: information-enzyme activity determination method to solve the dependence of enzyme activity determination is way for standardization is the best way to solve the problems. Early in the 20 years ago international began work on that. At the time of the proposed standardized way recommend using uniform determination method, some academic group launched a clinical often  catalytic activity determination of the recommended and reference method. After years of hard work, to improve the enzyme success by quality and comparability. But the effect is not satisfactory, there is also some more serious problem:

First, a lot of academic organizations proposed recommendation methods, even if the same principle, but reagent formula (buffer, substrate, additive, etc.), reaction temperature and so on also may be different, so the lack of consistency between different recommended;

Second, recommend to keep up with the analysis method and the analysis of technical progress;

Third, it does not adapt to the automatic analysis instrument.

Recent international put forward the determination of enzyme activity standardization new ways to use is recognized as one of the enzyme calibration substances that different conventional methods to get the measured results of unity. At present the study has made some progress.
By using enzymes that method is to the calibration of the enzyme calibration substances that recognized one or more conventional methods to determine the results and the same standard method, also is through the enzyme calibration things will be different methods of result of the numerical value of things according to the standard determination value selected morning standard method to express. It is to use standard content as the carrier, make the determination results in the standard method and numerical transmission between conventional methods. So, to use the standard calibration thing, it is possible to unified the determination results.

Each enzyme has two laboratory in the study, all use the same kit in the automatic was on. The use of the calibration laboratory are unified content. Through the use of the enzyme, conduct unified calibration calibration, can make the different temperature (30 ℃, 37 ℃), use different buffer determination, different substrates measured or different substrate concentration determination of the kit relatively concentrated determination results.

The health ministry clinical inspection center in 1997 in automatic biochemistry analyzer users in the use of unified calibration matter tested before the serum calibration quality of the investigation. The laboratory for part of the automatic biochemistry analyzer users. The users with the use of the kit for routine use of their ordinary kit, most for products. The test results show that the distribution of the determination results after calibration standard must focus on significantly better than before, and a calibration more close to the serum levels of quality before target value, show that using uniform calibration product can make use of different determination of enzyme activity of the reagent relative reunification, increased the comparability between different lab results. But some of the calibration laboratory after its determination results are still very dissatisfied, this could be used by these laboratory the measurement method of the specificity and calibration of things fixed value the method is different, also can not completely ruled out qc serum effect of matrix.
To be through this way to get the same enzyme activity determination result, there are two conditions must meet the:
The first is the principle to ensure consistent determination method is to standard the conventional method and the specificity of the same method as far as possible. The common method is to use the proof of the reference method and the conventional method and patients were sample (more than 50 cases, a wide range of distribution, observing the relationship between two methods.

The second condition is enzyme calibration convertible sex thing. The use of the enzyme that in standard method must be calibrated and the calibration of the conventional method have the same characteristics. That is the calibration of the matrix effect should be very small things.
                            Article: From perlong medical (http://www.pl999.net/)

Use of automatic blood analyzer to detect blood sugar technology research

 IV.Dynamic blood glucose monitoring technology
 Because of biochemical analysis and fast blood cells analyzer and this minimally invasive technique can't or continuous dynamic detection of patients with glucose in the blood and can't get closer to the truth so, dynamic blood glucose monitoring system (CGMS) came into being dynamic spectrometry can eliminate individual differences and measuring conditions on the influence of the spectral measurement.

 Studies have think that, as a result of the pulse of the phenomenon, blood vessels in blood flow to a periodic change, blood is not transparent liquid, the light in the blood, penetrating than in the organization through small a few times, so the change may cause the pulse of near infrared spectral absorbency change, so through the dynamic spectrum record artery to maximum and filled with the arterial constriction of the most hours to spectrophotometry value, can remove the individual differences and measuring conditions on the influence of the spectral measurements, correction model prediction ability, improve the precision of the spectral measurement MiniMed company production of the United States CGMS already in June 1999, the FDA approved for listing.

 CGMS is a minimally invasive blood glucose monitoring system, by detecting subcutaneous tissue of the liquid glucose concentration between and reflect the blood sugar level, it will continuously monitoring patient 1 day at each hour of the blood glucose levels, this instrument is only the size of a mobile phone, with microcomputer chip, there is a slight hose connection instruments and detection head, detection head into the subcutaneous tissue every 10 seconds from detector instrument to an electrical signal changes reflect blood sugar, the electric signals of every 5 minutes average conversion to blood sugar leels storage up every day can record store 288 blood sugar value the instrument and can also record and storage dine sports events such as drug use.
 CGMS to continuous monitoring three days (72 hours) the dynamic change of blood sugar, and then the data can be downloaded to the ordinary computers, provide to the doctor diagnose evidence however, CGMS and non-invasive detection techniques of GWG2B device, accuracy also there are some problems, mainly in induced insulin hypoglycemia, these devices monitor blood glucose is not high credibility, therefore, the food and drug administration suggest not only the material and change the treatment, must first use standard of blood glucose meter checked.
 To sum up, and the technology and noninvasive technique each have advantages and disadvantages, in a long stage, two kinds of technology will complement each other, common development, but diabetes diagnosis must through hematology analyzer of venous blood samples analysis analyzer glucose levels as standard, noninvasive and continuous dynamic type the accuracy of the glucose monitor must be tested periodically correction.
 In short, and the blood glucose testing technology development or minimally invasive more mature, although in recent years the noninvasive and continuous dynamic type blood sugar test technology develops very quickly, but still needs to be used to continue research to improve the radio waves and microwave technology measure glucose content is also being studied believe that with the rapid development of science and technology, blood glucose detection technology will quickly and accurately to improve simple direction

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Biochemistry Analyzer Reagents Kits

Biochemistry Analyzer Reagents Kits
Specifications
A,Fully-automatic package for Hitachi 7170/7080/7180/7600,OLYMPUS400/640/2700;Keysys
B,Special package for Hitachi7150/7060; Olympus1000; Toshiba120FR; Abbott2000/8000; Shimadzu7200/7300,Hitachi7150/7060
C,Specialize for Hitachi7020 biochemistry analyzer
D,Specialize for Beckman: CX3/CX4/CX5/CX7/CX9/LX20 biochemistry analyzer
E,Specialize for Toshiba-30/40 FR biochemistry analyzer

Urine Chemistry analyzer validation patients and glycemic load the uric acid

      Experts in through the with blood sugar and high blood pressure patients uric acid test research shows that uric acid in patients with high blood pressure is normal sugar and 1 hour after glycemic load significant correlation.
      An oral glucose tolerance test (OGTT) 1 hour quartile blood sugar 155 mg/dl can determine the risk of type 2 diabetes and the clinical organ damage NGT of subjects. Even so, still be classified as low NGT individuals threatened the crowd. High uric acid with high blood pressure, blood insulin resistance and diabetes and several kinds of cardiovascular disease risk related. But it is still not clear uric acid in hypertensive NGT individuals can affect blood glucose after 1 hour load. The study participants included 955 patients with hypertension outpatient service of OGTT routine. Laboratory index is to evaluate and estimated the estimated glomerular filtration rate.
     The results showed that uric acid in the whole crowd for 1 hour after the main prediction blood sugar load factors; In a separate model, NGT quartile 155 mg/dl subjects, glucose tolerance and damaged in patients with type 2 diabetes accounted for 26.0% of the variation, 25.3%, 13.5% and 13.5%.


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The maintenance of the biochemistry analyzer

    

        There are many kinds of the biochemistry analyzer.The can be classified  into two groups,semi-automatic biochemistry analyzer and automatic biochemistry analyzer.
        And about the maintenance of the two kinds of the biochemistry analyzers are the same. When the biochemistry analyzer is in the course of using normally, it only needs the normal clean and maintenance, but the follow components with long time work may be invalid or fault, at this moment you should mend it to the appointed maintenance point or under the professional technician guiding.  
 

Now,let see some parts of the biochemistry analyzer .

 Filter 
 Through a period of using time, the transmissivity of filter will reduce. It reduces until instrument cannot normal work, instrument will prompts automatically, at this moment in order to guarantee the accuracy of detecting, you should invite professional technician to replace the invalid filter.

Lamp-house light
 When lamp-house light is defective (not light), instrument will prompt automatically, and at this moment you open the above cover to check-up. If confirming the light defective, you should replace it by the same type of light, and if power supply fault, you repair it.

 

 Pumpline
    Through a period of using time, because of extrusion, the pumpline may be distortion to influence of the aspiration volume. Before detecting, you should check-up whether pumpline is distortion, and if it is, please replace it.

Fuse
   After fuse burnt out, you must cut off power supply firstly, and then replace fuse by the same type, or you do the above operation under professional technician guiding.

    If the maintenance of the biochemistry analyzer is very good,then you will use the biochemistry analyzers for a long time.

Clinical lab device in perlong medical : PUS-2018G semi-auto chemistry anlayzer

Biochemistry Analyzer
 Biochemistry Analyzer(PUS-2018G ) is one of the clinical laboratory devices .
 The biochemistry analyzer is semi-auto biochemistry analyzer.
The brand of this biochemistry analyzer is Perlong.

Usage of biochemistry analyzer (PUS-2018G )
       The biochemistry analyzer is a high performance photometer and can be used in the clinic of the hospitals,epidemic prevention stations and health protection stations for biochemical test.


Technical parameters  of biochemistry analyzer (PUS-2018G )

Wavelength range:340nm~750nm
Measurement range:0.100~3.500Abs
Test Method: Absorbency, End-poit, Kinetic, Two-point
Colorimetric Pool: Titanium alloy

Features of biochemistry analyzer
1.The biochemistry analyzer can do detection, program and enquiry as well as drawing quality control curve.
2. Showing and printing calibrating curve, Kinetic reaction curve, and print quality control curve.
3.The biochemistry analyzer has the function of monocrotic and dual wavelength detection;
4.Detected by the means of absorbance, kinetics, terminal, two-point, standard curve, blank comparing pipe and factor. It can print single report in real time and general report.
5.Auto-check first after it is switched on and it also can communicate with the computer.
6.The biochemistry analyzer has half press dormancy function.