2012年7月25日星期三

five kinds of blood coagulation analyzer immunological method

 Blood coagulation analyzer referred to as "blood clots device, in blood clotting immunological method in the analyzer of purification for material as the antigen, corresponding antibody preparation, then use the antigen antibody response to be seized for qualitative and quantitative determination. Commonly used method have five:

First, blood coagulation analyzer rockets electrophoresis
In certain electric field, gel to support within the things their corresponding antibody detection was formed by the combination of a "rocket peak", the rockets the height of the peak is proportional to its content, by means of measuring the high peak and compare with standard quantitatively determined. In this operation complex, less clinical application.
Second, blood coagulation analyzer two-way immune electrophoresis
Through the level and vertical two direction electrophoretic will some molecular structure of abnormal blood clot separation.

Third, blood coagulation analyzer ELISA test (ELISA)
YongMei standard antigen and antibody and were seized on the antigen reaction, after washing to remove not combination of antigen or antibodies and the specimens interfering substances, leaving fixed in wall antigen antibody complex, and then join the enzyme substrates and color of the original material, the reaction that produces the non-ferrous material, standard instrument determination, the color depth and were seized in the concentration ratio relations. The high sensitivity, the specific strong, has used in many stop bleeding, thrombus composition detection.
Fourth,semi-auto blood coagulation analyzer immune diffusion method
Will be seized and corresponding antibodies in certain media union, determine the precipitation of the size of the ring, and compare with standard, the calculation of the concentration of the articles to be tested. In this operation is simple, do not need special equipment, but too time consuming, and the sensitivity is not high, is only suitable for content is higher blood coagulation factor of blood clots analyzer test.
Fifth, blood coagulation analyzer immune than turbidity method
Will be seized and its corresponding antibody a complex mixture, which generate enough precipitation grain, through the transmission than muddy or scattering determined than polluted. In this operation is simple, accuracy, good, facilitate automation.
Article: from perlong medical (http://www.pl999.net)
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