2012年7月25日星期三

Microplate reader to bacteria levitation liquid OD value is determined

Microplate reader in microbiology application specific performance of bacteria in the determination of value for liquid OD, perlong medical equipment expert analysis specific performance shown below:
Growth curve generally refers to total bacterial colonies CFU/mI. Or the logarithm of total bacterial colonies log CFU/ml. Changes with time curve, can be used to display microbial growth and metabolism rule. Because of the tested bacteria number/concentration and suspension of the optical density bacteria liquid (optical density, OD) is proportional to the values, so the growth curve are also used OD-t curve said.
At present bacteria levitation liquid OD is mainly through the visible spectrophotometer to measure, its evaluation microbial growth in state can obtain satisfactory results. But this method has a certain limitation:
1, real-time poor;
2, should be repeated sampling, the operation tedious;
3, sampling and testing process medium to easily infect miscellaneous bacteria;
4, as XuanZhuoYe, the bacteria have certain sedimentation rate, detection of the length of time OD value produces an effect, makes the experiment reproduce more difficult.
Due to the mciroplate reader is a kind of fast, accurate, high throughput of medical equipment, so we always choose to use enzymes standard instrument for bacteria levitation liquid OD the determination of value, to improve work efficiency and save time.
In addition to determine bacteria levitation liquid OD value outside, auto elisa reader also can be used to detect the concentration of bacteria. For example: various kinds of textiles close relation with human life, its surface attached microbes may be the spread of the disease and the important media or body accompanied by microbes formed a kind of organic micro ecology relationship. With the development of The Times, all kinds of anti-bacterial fabric product appear constantly, but for evaluation of antimicrobial textiles.
The bacteriostatic effect, there is no standard methods, so, the establishment of a scientific evaluation method of fabric antibacterial effect health microbiology and micro ecology studies have important value. Because the living bacterium count much error and the work tedious and do not apply to the mass of the specimen test, so use auto micropalte reader this medical device testing bacteria concentration we can establish a detection weaving bacteriostatic effect of the effective method.
Article: from perlong medical (http://www.pl999.net)
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