We all know that
blood coagulation
analyzer are blood clotting instrument, blood clots analyzer
biological chemical method is by measuring the substrate in the color that
change spectrophotometry test material content and activity of, this method can
also be called as the substrate show color method.
Blood coagulation analyzer in use color substrate detection thrombosis and hemostasis index of the principle is: through the synthetic and natural clotting factors have similar a amino acid sequences and there is particular role of sites small peptides, and can produce the color the hydrolysis chemical genes and sites linked to the role of amino acids. Determination because clinical blood coagulation factor has protein don't understand of the enzyme activity, it not only action natural protein peptide chain, also can effect of peptides in synthetic substrate, thus release produce color genes so that the solution is color. Produce color depth and blood coagulation factor activity is proportional, it can be accurate quantitative. At present synthetic peptide substrate has a few kinds, but the most common is p-nitroaniline (PNA), yellow, can use 405 mm wavelength method.
Perlong medical equipment experts point out that blood coagulation analyzer biological chemical method often use the following three forms:
First, first will be in plasma some enzyme screening to activation and thus activate the clotting factors of synthetic substrate for the hydrolysis is color, such as fibrinolytic enzyme original, determination of protein C.
Second, to be seized plasma add too much about reagents, and corresponding to the anticoagulation factor, then determined the residual the enzyme activity, such as AT-activity determination, and alpha 2-anti fibrinolytic enzyme determination, the determination of heparin.
Auto Blood coagulation analyzer biological chemical method high sensitivity and precision, and easy to automation, for thrombosis/hemostatic detection open up new ways.
Article: from perlong medical (http://www.pl999.net)
Attention: if you want to copy this article , you must attach the website.
Blood coagulation analyzer in use color substrate detection thrombosis and hemostasis index of the principle is: through the synthetic and natural clotting factors have similar a amino acid sequences and there is particular role of sites small peptides, and can produce the color the hydrolysis chemical genes and sites linked to the role of amino acids. Determination because clinical blood coagulation factor has protein don't understand of the enzyme activity, it not only action natural protein peptide chain, also can effect of peptides in synthetic substrate, thus release produce color genes so that the solution is color. Produce color depth and blood coagulation factor activity is proportional, it can be accurate quantitative. At present synthetic peptide substrate has a few kinds, but the most common is p-nitroaniline (PNA), yellow, can use 405 mm wavelength method.
Perlong medical equipment experts point out that blood coagulation analyzer biological chemical method often use the following three forms:
First, first will be in plasma some enzyme screening to activation and thus activate the clotting factors of synthetic substrate for the hydrolysis is color, such as fibrinolytic enzyme original, determination of protein C.
Second, to be seized plasma add too much about reagents, and corresponding to the anticoagulation factor, then determined the residual the enzyme activity, such as AT-activity determination, and alpha 2-anti fibrinolytic enzyme determination, the determination of heparin.
Auto Blood coagulation analyzer biological chemical method high sensitivity and precision, and easy to automation, for thrombosis/hemostatic detection open up new ways.
Article: from perlong medical (http://www.pl999.net)
Attention: if you want to copy this article , you must attach the website.
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