The determination
ofelisa reader
wavelength range has single wavelength and double wavelength
division, specific content as follows:
General elisa reader of medical devices determination in 400 ~ 750 nm wavelength or between 800 nm, and can meet the ELISA show color measurement. At present domestic common ELISA kit use YongMei mark are horseradish peroxidase (HRP), the substrate is usually four methyl LianBenAn (TMB) and adjacent benzene 2 amine (OPD), the hydrogen peroxide solution under the existence of, the HRP role, respectively for 2, 2 oxidation,-two amino azo benzene (DAB) and bifenthrin hydroquinone.
When the pH value of about 5.0, DAB in the 450 nm wavelength place has the maximum absorption, when pH value reduced to L 0, the maximum absorption wavelength moved to 492 nm, and Moore the extinction coefficient change, show color deepened, so commonly used acid as sulfuric acid or hydrochloric acid terminated reaction. TMB oxidation product PCBS in 450 nm wavelength) place has the biggest extinction coefficient, if HRP quantity is little, H: O: and TMB excessive is forming the blue cationic root. Lower pH, can make blue cationic root into yellow biphenyl quinone, using sulfuric acid as termination agent can make product stability 90 min.
Therefore, 450 nm and 492 nm two wavelengths is the most commonly used at present ELISA determination. Various medical equipment auto microplate reader is equipped with placed filter can be automatically convert parts, can also install 6 ~ 8 pieces of filter, equipped with the filter should be included in the above two wavelength, some enzymes to 490 nm standard instrument filter alternative 492 nm filter, the impact is not big. In addition to the two basic filters outside, considering the needs of double wavelengths than color, also should have the 620 nm 630 or nm 650 or nm and 405 nm wavelength of filter, other filter can choose according to their own needs. Sometimes, some hope standard instrument for laboratory trace biochemical determination, the enzyme standard instrument manufacturers to its production of microplate reader expanded the ultraviolet detection function, this is a place for a 340 nm wavelength filter. At this time, the determination of auto elisa reader wavelength range will be 340-750 nm 800 or nm.
Standard of medical equipment instrument enzyme has single wavelength and dual wavelength detection function. Sometimes the user never know under what circumstances use single or dual wavelength detection. The so-called "single wavelength" is to use a kind to have the biggest show color absorbed wavelength or 450 nm 492 or nm to colorimetric determination.
Article: from perlong medical (http://www.pl999.net)
Attention: if you want to copy this article , you must attach the website.
General elisa reader of medical devices determination in 400 ~ 750 nm wavelength or between 800 nm, and can meet the ELISA show color measurement. At present domestic common ELISA kit use YongMei mark are horseradish peroxidase (HRP), the substrate is usually four methyl LianBenAn (TMB) and adjacent benzene 2 amine (OPD), the hydrogen peroxide solution under the existence of, the HRP role, respectively for 2, 2 oxidation,-two amino azo benzene (DAB) and bifenthrin hydroquinone.
When the pH value of about 5.0, DAB in the 450 nm wavelength place has the maximum absorption, when pH value reduced to L 0, the maximum absorption wavelength moved to 492 nm, and Moore the extinction coefficient change, show color deepened, so commonly used acid as sulfuric acid or hydrochloric acid terminated reaction. TMB oxidation product PCBS in 450 nm wavelength) place has the biggest extinction coefficient, if HRP quantity is little, H: O: and TMB excessive is forming the blue cationic root. Lower pH, can make blue cationic root into yellow biphenyl quinone, using sulfuric acid as termination agent can make product stability 90 min.
Therefore, 450 nm and 492 nm two wavelengths is the most commonly used at present ELISA determination. Various medical equipment auto microplate reader is equipped with placed filter can be automatically convert parts, can also install 6 ~ 8 pieces of filter, equipped with the filter should be included in the above two wavelength, some enzymes to 490 nm standard instrument filter alternative 492 nm filter, the impact is not big. In addition to the two basic filters outside, considering the needs of double wavelengths than color, also should have the 620 nm 630 or nm 650 or nm and 405 nm wavelength of filter, other filter can choose according to their own needs. Sometimes, some hope standard instrument for laboratory trace biochemical determination, the enzyme standard instrument manufacturers to its production of microplate reader expanded the ultraviolet detection function, this is a place for a 340 nm wavelength filter. At this time, the determination of auto elisa reader wavelength range will be 340-750 nm 800 or nm.
Standard of medical equipment instrument enzyme has single wavelength and dual wavelength detection function. Sometimes the user never know under what circumstances use single or dual wavelength detection. The so-called "single wavelength" is to use a kind to have the biggest show color absorbed wavelength or 450 nm 492 or nm to colorimetric determination.
Article: from perlong medical (http://www.pl999.net)
Attention: if you want to copy this article , you must attach the website.
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