2012年9月6日星期四

Four different functions microplate readers

Microplate washer, microplate reader and computer components make up the ELISA test workstation, which microplate reader can be divided, according to function, into four microplate medical devices, namely light absorption microplate fluorescence microplate reader chemiluminescence microplate reader and multifunctional microplate reader.
A first light absorbing microplate reader
The light absorbing microplate is used for the visible and UV absorbance detection, a specific wavelength of light through the sample in the microplate, the light energy is absorbed, the light energy is absorbed and the concentration of the sample was a certain proportional relationship , and thus can be used to qualitatively and quantitatively detecting the light absorption detection of mature technology, low cost, simple operation, but the dynamic range is narrow, relatively low sensitivity, specificity is not strong. Generally visible and ultraviolet light, respectively, using a tungsten lamp and deuterium lamp as a light source, and UV / Vis microplate reader can be two sources to switch to adapt to the needs of the different measurement wavelength.
Second, fluorescence microplate reader
The fluorescence microplate reader is used for the detection of the fluorescence incident on the calibration of the fluorescent substance on the sample by the excitation light of a specific wavelength in the grating, will issue a longer wavelength emitted light to reach the detector, by the emission of the grating. The fluorescence intensity and the concentration of the sample was a certain proportion of the high sensitivity of fluorescence detection, real-time detection, easy to use, diversity detection mode, but susceptible to outside interference, exciting and emitted light is likely to influence each other, interference detection.
Third, chemical luminescence microplate reader
Chemiluminescence microplate reader from biochemical reactions in the self-luminous glow and flash two types can be divided. Glow-type luminescence lasting, stable, and can continue for some time; short flash-type light-emitting time changes quickly, the stability is not strong, the automatic injector needs to be applied before they can proceed. Was a certain ratio between the number of photons emitted in the chemiluminescence sample volume, chemiluminescence microplate reader sensitivity is very high and wide dynamic range.
Fourth, multifunctional elisa reader
Multifunctional microplate reader these three microplate reader collection, it integrates two or three microplate reader in one, both light absorption, fluorescence and chemiluminescence detection, powerful, and the use of a wide range of as a research platform, to avoid a repeat purchase, is the first choice for the laboratory. In addition, according to the number of channels, the microplate reader is divided into two types of single-channel and multi-channel, single channel there are both automatic and manual divided.
The above information is for reference only!

Article : From Perlong medical (http://www.pl999.net/)
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