No matter in the
purchase microplate
reader or washing machine, enzyme from inspection workstation are
need to have a perceptual knowledge, from the medical instrument appearance,
absorbance measurement repeatability, linearity, stability and accuracy to
judge, the concrete content shown below:
First, the appearance
Clinical Microplate reader should instrument name, model, Numbers, manufacturers, production date and power supply voltage; The adjusting knob, keys and switch all can work normally, outside surface no obvious mechanical damage; Display text should be clear complete.
Second, absorbance measurement repeatability
Wavelength choice with (3), in the enzyme label plate first row charging blank solution, the second row join concentration for 200 rig/ml of potassium dichromate solution, repeated measurement 5 times. Record every time determination of the second row of absorbance value, take the second row RenYiKong absorbance value and every corresponding hole absorbance value. Press type calculation repeatability:
Third, clinical laboratory microplate reader for laboratory linear
Choose 540 nm wavelength, the nominal value o. 5,1.0 A neutral filter respectively in the special test plate sample position, air as reference, the determination of repeat 3 times; Then the above two pieces of neutral filter stack in special test board after the same hole location, determination of repeated three times. Linear error:
Type of A1 for the first piece of neutral filter absorbance mean, A: for the second piece of neutral filter absorbance mean, A1, 2 for two pieces of neutral filter stacked absorbance mean.
Article: from perlong medical (http://www.pl999.net)
Attention: if you want to copy this article , you must attach the website.
First, the appearance
Clinical Microplate reader should instrument name, model, Numbers, manufacturers, production date and power supply voltage; The adjusting knob, keys and switch all can work normally, outside surface no obvious mechanical damage; Display text should be clear complete.
Second, absorbance measurement repeatability
Wavelength choice with (3), in the enzyme label plate first row charging blank solution, the second row join concentration for 200 rig/ml of potassium dichromate solution, repeated measurement 5 times. Record every time determination of the second row of absorbance value, take the second row RenYiKong absorbance value and every corresponding hole absorbance value. Press type calculation repeatability:
Third, clinical laboratory microplate reader for laboratory linear
Choose 540 nm wavelength, the nominal value o. 5,1.0 A neutral filter respectively in the special test plate sample position, air as reference, the determination of repeat 3 times; Then the above two pieces of neutral filter stack in special test board after the same hole location, determination of repeated three times. Linear error:
Type of A1 for the first piece of neutral filter absorbance mean, A: for the second piece of neutral filter absorbance mean, A1, 2 for two pieces of neutral filter stacked absorbance mean.
Article: from perlong medical (http://www.pl999.net)
Attention: if you want to copy this article , you must attach the website.
没有评论:
发表评论