2012年9月5日星期三

Software is the Only Standard to Judge Whether Microplate Reader is Good or not

The software has become the only indicator to determine a category of medical devices. The following is an analysis microplate reader software features.
The software functions on ELISA microplate reader has the qualitative and quantitative determination and other measurement methods such as ELISA kinetics, ultraviolet, and agglutination of statistical data analysis and report results of the function.
The software features a very important function in upscale microplate reader. If the hardware side is not much different, the software to determine the the microplate reader the merits of the only indicators. For users, a good software functions, the actual work will be a great help. ELISA qualitative determination, microplate reader as a positive judgment value (cut-off) and the determination of the "gray zone" (ie absorbance was measured in a certain area around the cut-off, the results should be within this area as "suspicious") statistics calculation functions, not only to facilitate the laboratory staff, and, in certain cases, a high practical value. Blood bank laboratory for screening blood donors blood of HBsAg, anti-HCV and anti-HIV ELISA, often encounter the absorbance was measured at a cut-off near but below the cut-off of blood, according to the kit's standard can be judged as negative blood for qualified blood, can be used in patients with transfusion, but intuition tells us that this blood, such as to the patient, leading to the possibility of the disease after transfusion Determination by ELISA the uncertainty caused by, that is, the "gray zone", the presence of the ELISA assay makes ELISA assay kit the setting of the cut-off only relatively accurate, lower the possibility of the occurrence of false positive and false negative. Therefore, blood donors, blood in the blood bank screening, such as the determination of the lower limit of the "gray area" of the blood screening blood donors as a judge fail criteria, can be avoided due to the "gray area" due to the result of an error of judgment and cause disease after transfusion likelihood of occurrence. Appropriate software functionality is also very important for ELISA quantitative determination. The ELISA test workstations experts studies have shown that the four-parameter equation can better reflect the immunoassay dose-response curve, and the most suitable for quantitative enzyme immunoassay curve fitting. Therefore, if the purpose of the quantitative determination of the microplate reader software features, it is best to have this curve regression equation analysis capabilities. About others, such as points, lines, etc. regression calculation can microplate reader application purpose, such as part-used to trace biochemical determination of such regression calculation is necessary.
Microplate reader cater to ELISA dynamics agglutination determination of the required software should have, of course, should be based on the purpose of use. Such software are generally more expensive, microplate reader often separately on demand with, if not the special needs of the laboratory, do not insist on this software.

Article : From Perlong medical (http://www.pl999.net/)
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