Attention of using biochemical analyzer

Correct operation of biochemical analyzer the accuracy of measurement results is very important material calibration reagent operation method, the results of calibration, the test results are certain influence in order to correctly use biochemical analyzer, guarantee the accuracy of the test results we summarize the following tips, only for the operator and maintenance engineers reference.
I.  According to the regulations operation, maintenance regularly maintenance equipment
According to provisions of operation procedure, according to the requirements in time, maintenance, maintain week, month quarter maintenance special attention when a test project turned to another test project, must be timely cleaning than color cup, will the remnants of reaction fluid rushs, otherwise it will cause the test result error
II.  According to the rules set parameters reagents used
Use of the reagents must be within the warranty period, reagent general kept in 2-8 under temperature must press reagents required input that set parameters to ensure accurate test, pollution can increase the reagent absorb fluid amount, generally absorb fluid amount should be more than 400 μ l, general project for 500 μ l, maximum available to 700 μ l to ensure that cross contamination rate < 1%
III.   Handle the sample correctly
General requirement samples:
According to the requirements of the sample pretreatment and save the sample
2.Samples are not allowed to have clot, shall not be turbid
3. Samples of some material: such as drug anticoagulation preservatives cannot affect the measurement
4. Samples for volatile can no longer open cover placed
5. Hemolysis jaundice chylothorax particles can influence measurement results, suggest blank
IV.Choose the correct test methods and set delay time
Biochemical tests have many kinds of methods, general use a method of the rate method two points and so on delay time is to point to the measured liquid into than color cup up, to the actual testing begins to in order to make the time for the specimen than color in the pool of temperature balance and tiny bubbles disappear, certain delay is necessary general most end to delay test method for 5 seconds, and rate method in under 25, delay should be not less than 10 seconds, in under 30 delay should be not less than 12 seconds, and 37 the following delay should be not less than 15 seconds in addition, generally with the two points can choose reagent blank, and rate method does not establish a blank
V.   A complete quality control
Usually biochemical analyzer measurement is the reaction liquid spectrophotometry value, and the operator hope to get is chroma value, it will need to test one or more of the concentration of the reaction of the liquid known spectrophotometry value, to a conversion factor through the conversion factor for the reaction he can be the liquid spectrophotometry value conversion for density of the concentration of the known reaction liquid called standard samples, through the standard material for the son get conversion process known as calibration so to get the correct conversion factor, measurement accurate and reliable must use reliable standard material and the correct calibration so must use reliable standard content and standard quality and prior input parameters and can be used for testing instrument to test the integrity of the instrument must be accurate in the lab