In recent years, with multi-function microplate reader in the domestic various universities laboratory gradually promotion to open, and multi-functional microplate reader brands and models also gradually more up, gradually to spend your eyes in addition to each brand all have their own a even more series product line, character of each are not identical, when the choose and buy all kinds of technical parameters technology index is mind-boggling.
This paper try to from the user of the actual use of the perspective, probes into how to look at the great variety of characteristic parameters, the hope can help you find the real appropriate oneself more reactive enzyme from inspection workstation.
I. Filter Vs grating
Multifunctional microplate reader classification method is numerous, but the most simple is using their filter way to make light boundaries in general, could be divided into filter type and grating type two kinds big but filter and grating and can't finish at the same time the same testing, or want to use when the grating with grating, the use of the filter with the filter; And some of the experiments with one of them not, and the other a module that didn't happen so this kind of instrument is essentially the filter and grating in together, and not make the two produced the new breakthrough mix.
Overall, filter technology because development already for a long time, with two to color mirrors (in fact also is another mode filter light reflective filter) light path system, can realize the need to present the most conventional multi-function enzyme standard instrument of the highest detection sensitivity is to use the filter to make but type filter type instrument because of limited to filter wavelengths and limit, can't satisfy the growing experiment types of detection will need, and sometimes need to the absorption of material excitation and emission spectrum research, so later was born grating type instrument.
Grating is the advent of the standard type enzymes, and the user in the wavelength choice no longer restricted, and in the miscellaneous light rate control performance bandwidth is beyond the filter system these are currently filter microplate reader cannot or more difficult to achieve.
II . Miscellaneous light rate and wavelength accuracy
Grating type filter system has become the general current peremptory multi-function microplate reader of mainstream, several manufacturers joint efforts, has set the grating technology driven to a record high in many of the technical parameters of grating, the key is no doubt of the grating miscellaneous light rate and the accuracy of the wavelength selection.
Miscellaneous light the light source is the rate through the grating, get light, do not need to account for the wavelengths of light that the proportion of nominal wavelengths of light, and characterization of the purity of the filter light because the light interference of the complexity of the diffraction, no matter use filter or grating, miscellaneous light is inevitable all kinds of filter the essence of technology is light to try to remove noise as possible light.
Generally speaking, filter type of noise rate in light 10-4 ~ 10-5 between, grating type can do 10-6 ~ 10-seven because this kind of miscellaneous light nonspecific, but also directly into the last of the detector, so how many mixed the light will how much random errors introduced in fluorescence detection such as process, because detector amplification effect exists, noise interference light rate will also be index amplifier therefore, miscellaneous light rate is a filter system's first performance indicators
The two , it is grating even filters filter system the most key technical parameters, directly affect is to get data is really to the results of the measurement, the reliability of the experimental results is the most basic requirements.
Article: from perlong medical (http://www.pl999.net/)
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