the study of the method of enzyme activity : Song typeface blackbody enzyme from the inspection workstation biochemical analyzer determine

Perlong medical report: information-enzyme activity determination method to solve the dependence of enzyme activity determination is way for standardization is the best way to solve the problems. Early in the 20 years ago international began work on that. At the time of the proposed standardized way recommend using uniform determination method, some academic group launched a clinical often YongMei catalytic activity determination of the recommended and reference method. After years of hard work, to improve the enzyme success by quality and comparability. But the effect is not satisfactory, there is also some more serious problem:
First, a lot of academic organizations proposed recommendation methods, even if the same principle, but reagent formula (buffer, substrate, additive, etc.), reaction temperature and so on also may be different, so the lack of consistency between different recommended;
Second, recommend to keep up with the analysis method and the analysis of technical progress;
Third, it does not adapt to the automatic analysis instrument.
Recent international put forward the determination of enzyme activity standardization new ways to use is recognized as one of the enzyme calibration substances that different conventional methods to get the measured results of unity. At present the study has made some progress.
By using enzymes that method is to the calibration of the enzyme calibration substances that recognized one or more conventional methods to determine the results and the same standard method, also is through the enzyme calibration things will be different methods of result of the numerical value of things according to the standard determination value selected morning standard method to express. It is to use standard content as the carrier, make the determination results in the standard method and numerical transmission between conventional methods. So, to use the standard calibration thing, it is possible to unified the determination results.
Each enzyme has two laboratory in the study, all use the same kit in the automatic was on. The use of the calibration laboratory are unified content. Through the use of the enzyme, conduct unified calibration calibration, can make the different temperature (30 ℃, 37 ℃), use different buffer determination, different substrates measured or different substrate concentration determination of the kit relatively concentrated determination results.
The health ministry clinical inspection center in 1997 in automatic biochemistry analyzer users in the use of unified calibration matter tested before the serum calibration quality of the investigation. The laboratory for part of the automatic biochemistry analyzer users. The users with the use of the kit for routine use of their ordinary kit, most for products. The test results show that the distribution of the determination results after calibration standard must focus on significantly better than before, and a calibration more close to the serum levels of quality before target value, show that using uniform calibration product can make use of different determination of enzyme activity of the reagent relative reunification, increased the comparability between different lab results. But some of the calibration laboratory after its determination results are still very dissatisfied, this could be used by these laboratory the measurement method of the specificity and calibration of things fixed value the method is different, also can not completely ruled out qc serum effect of matrix.
To be through this way to get the same enzyme activity determination result, there are two conditions must meet the:
The first is the principle to ensure consistent determination method is to standard the conventional method and the specificity of the same method as far as possible. The common method is to use the proof of the reference method and the conventional method and patients were sample (more than 50 cases, a wide range of distribution, observing the relationship between two methods.
The second condition is enzyme calibration convertible sex thing. The use of the enzyme that in standard method must be calibrated and the calibration of the conventional method have the same characteristics. That is the calibration of the matrix effect should be very small things.

                                           Article: From perlong medical