2012年3月6日星期二

Matters need attention in the microplate reader instrument research detection of myohemoglobin

Perlong medical equipment company red protein is the popular science muscle of a kind of lay aside oxygen protein. By 153 amino acid residues composition, containing hemoglobin, and hemoglobin homologous, and the combination of oxygen ability between hemoglobin and cytochrome oxidase between, can help muscle cells will oxygen transfer the mitochondria. Through the enzyme standard instrument research found that it is by a peptide chains and a hemoglobin, the combination of auxiliary of protein, is muscle in the oxygen storage protein, it of oxygen saturation curve of the elliptic type.
1. Because there was also in skeletal muscle Mb, and only from glomerular filtrate removed, so acute muscle damage and various causes of patients with myopathy, long time of shock, acute or chronic renal full-time Mb will rise.
When Mb as early, quantitative diagnostic AMI biochemical marker except the above should be with the disease or disease.
2. Different manufacturers to the requirements of the specimen kits is different also, according to the requirements should take blood.
Such as the use of anticoagulants, usually by heparin anticoagulation.
With different analysis and detection technology income different reference value. The laboratory shall establish their respective range of all the reference.
3. An microplate reader check, because carbon anhydride enzyme isozyme Ⅲ does not exist in myocardial, mainly exists in skeletal muscle; And the release of the skeletal muscle from the mode and the same and Mb, so someone through the Mb/CA Ⅲ ratio to improve Mb diagnosis AMI sensitivity and specificity, studies show that Mb/CA Ⅲ AMI symptoms in two hours after he saw the rise, sensitivity and specificity are better than CK and CK Mb high, is also one of the markers of early myocardial injury.

Article: from perlong medical (http://www.pl999.net/)
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