2012年3月14日星期三

How to select microplate reader (2)

III. the authentication > parameters
       In order to ensure reliable detection, on the basis of the different instruments under a difference is reflected in the detection sensitivity on top, and then people care about is the instrument to detect more weak signal.
The light sensitivity involves road system design and material, and it's hard to say a certain components will be a decisive role but have a bit, in all kinds of ChanGongNeng testing project, light absorption detection with a large photoelectric diode put fluorescence detection with photomultiplier tubes with specially designed light detection single-photon counting photomultiplier tubes, these three different detector is their testing field choice of priority
       Each single detection of the optimal result is done with the corresponding detector, of course, also some instrument for cost consideration, use a variety of testing mode detector compatible, this in part to finish the experiment demand, but the performance will also have to sacrifice compromise, unable to realize all tests are to reach optimization.
        The definition and the detection sensitivity about standard, each manufacturer has its own description, will say that he is about how good, so it is difficult to have an intuitive objective is so, some reagent manufacturer stood out, the detection performance of some instruments to provide a third party certification.
        The instrument because new type grating technology in recent years that it has gradually arisen, limited to technology research and development and equipment costs, the same price range and sensitivity of index will be slightly worse than development mature filter machine so, in the midrange multi-function enzyme standard instrument field, got all kinds of authentication of the grating type instrument does not see more
        It shows that the type of meter standard grating enzyme development and to enter into a new height, except in the flexibility has an irreplaceable role outside, still in the detection sensitivity etc also gradually replacing the traditional filter type enzyme standard instrument grating is scientific research fields type multi-functional enzyme standard instrument future main trends of development; And filter type instrument is also to individual performance better impromotion.
IV  More than four color cup + trace detection
        In addition to regular 6 ~ 384/1536 hole enzyme panels detection besides, some with instruments than color cup trace detection board of compatible function whether used vertical than color cup than color glass or horizontal various trace detection board, the purpose is the process of light to not fixed enzyme panels detection into a standard optical path concept though enzyme panels detection can also through the optical path correction, etc. To realize 10 mm optical path OD value of the conversion, but it was just a mathematical conversion process, examination error accumulated more, actually use the effect not beautiful introduction and trace detection than color glass plate, light absorption of light by testing process fixed in 10 mm or the standard length of 0.5 mm, etc, OD value conversion more simple and accurate with such testing function of enzyme standard instrument, equivalent to in addition to the multi-function enzyme standard testing itself outside.

Article: from perlong medical (http://www.pl999.net/)
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